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bt549 breast cancer cell lines  (ATCC)


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    ATCC bt549 breast cancer cell lines
    Bt549 Breast Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2981 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bt549+htb+122+cell+lines/BT-549/pm42092157-225-10-32
    Average 99 stars, based on 2981 article reviews
    bt549 breast cancer cell lines - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.

    Fractionation:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.

    Incubation:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.

    Staining:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.

    Labeling:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.

    Microscopy:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.

    Control:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.

    SDS Page:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.

    Western Blot:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.

    Expressing:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.

    Activity Assay:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.

    Infection:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.

    shRNA:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.

    Sequencing:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.

    Immunoprecipitation:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.

    Knock-Out:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.

    CRISPR:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.

    Clone Assay:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.

    Quantitative RT-PCR:

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis.
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France), was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: CD36 enhances sensitivity of triple negative breast cancer cells to palmitate-induced ferroptosis
    Article Snippet: The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Ham’s F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 μg/mL hydrocortisone, and 5 μg/mL of human insulin.. The T47D (#HTB-133) and BT549 (#HTB-122) cell lines obtained from ATCC were maintained in RPMI 1640 medium supplemented with 10% ( v/v ) FBS (fetal bovine serum).. Cell lines were regularly checked for mycoplasma contamination.Cell lines were regularly checked for mycoplasma contamination.

    Article Title: ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
    Article Snippet: The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).The human pancreatic tumor cell lines NP9, NP18, and NP31 were established from xenografts orthotopically implanted in nude mice [ ] and were generously provided by Dr. Capella (Bellvitge Biomedical Research Institute-IDIBELL, Barcelona, Spain).. A549 (CCL-185) and BT549 (HTB-122) cell lines were obtained from the ATCC ® .. Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.Cells were maintained in culture for no longer than 3–4 months and cultured in 5% CO 2 and 95% air in a humidified atmosphere at 37 °C.



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    PAK2 expression and cluster formation in breast cancer cell lines. a mRNA expression levels of PAK2 in breast cancer cell lines and the normal mammary epithelial cell line MCF10A. b – f Quantification of cell cluster formation in four breast cancer cell lines under suspension culture conditions at 12, 24, and 48 h. g Immunoblot analysis of total PAK2 and phospho-PAK2 (Ser141) levels in <t>BT549,</t> MDA-MB-231, SKBR3, and AU565 breast cancer cells. SKBR3 cells were used as the reference (set to 1) for relative expression
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    Image Search Results


    PAK2 expression and cluster formation in breast cancer cell lines. a mRNA expression levels of PAK2 in breast cancer cell lines and the normal mammary epithelial cell line MCF10A. b – f Quantification of cell cluster formation in four breast cancer cell lines under suspension culture conditions at 12, 24, and 48 h. g Immunoblot analysis of total PAK2 and phospho-PAK2 (Ser141) levels in BT549, MDA-MB-231, SKBR3, and AU565 breast cancer cells. SKBR3 cells were used as the reference (set to 1) for relative expression

    Journal: Breast Cancer Research : BCR

    Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

    doi: 10.1186/s13058-025-02199-z

    Figure Lengend Snippet: PAK2 expression and cluster formation in breast cancer cell lines. a mRNA expression levels of PAK2 in breast cancer cell lines and the normal mammary epithelial cell line MCF10A. b – f Quantification of cell cluster formation in four breast cancer cell lines under suspension culture conditions at 12, 24, and 48 h. g Immunoblot analysis of total PAK2 and phospho-PAK2 (Ser141) levels in BT549, MDA-MB-231, SKBR3, and AU565 breast cancer cells. SKBR3 cells were used as the reference (set to 1) for relative expression

    Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

    Techniques: Expressing, Suspension, Western Blot

    PAK2 promotes cell cluster formation. a , b Immunoblot analysis showing PAK2 expression in BT549 cells after overexpression or knockdown. c Representative images and quantification of cluster formation in BT549 cells transfected with control vector (Con) or lenti-PAK2 (OE-PAK2). d Representative images and quantification of cluster formation in BT549 cells transfected with control siRNA (siCon) or siPAK2-1

    Journal: Breast Cancer Research : BCR

    Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

    doi: 10.1186/s13058-025-02199-z

    Figure Lengend Snippet: PAK2 promotes cell cluster formation. a , b Immunoblot analysis showing PAK2 expression in BT549 cells after overexpression or knockdown. c Representative images and quantification of cluster formation in BT549 cells transfected with control vector (Con) or lenti-PAK2 (OE-PAK2). d Representative images and quantification of cluster formation in BT549 cells transfected with control siRNA (siCon) or siPAK2-1

    Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

    Techniques: Western Blot, Expressing, Over Expression, Knockdown, Transfection, Control, Plasmid Preparation

    Effects of PAK2 on breast cancer cell progression. a , b Colony formation assays performed in BT549 cells with PAK2 overexpression or knockdown. c , d Wound healing assays evaluating the migration of BT549 cells with PAK2 overexpression or knockdown. e Transwell assays evaluating the invasive capacity of BT549 cells with PAK2 overexpression or knockdown

    Journal: Breast Cancer Research : BCR

    Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

    doi: 10.1186/s13058-025-02199-z

    Figure Lengend Snippet: Effects of PAK2 on breast cancer cell progression. a , b Colony formation assays performed in BT549 cells with PAK2 overexpression or knockdown. c , d Wound healing assays evaluating the migration of BT549 cells with PAK2 overexpression or knockdown. e Transwell assays evaluating the invasive capacity of BT549 cells with PAK2 overexpression or knockdown

    Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

    Techniques: Over Expression, Knockdown, Migration

    PAK2 mediates cell cluster formation via E-cadherin phosphorylation. a , b GSEA showing the association between PAK2 and the adherens junction pathway. c , d Immunoblot analysis of E-cadherin and phosphorylated E-cadherin (Ser840) in BT549 cells after PAK2 overexpression or knockdown

    Journal: Breast Cancer Research : BCR

    Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

    doi: 10.1186/s13058-025-02199-z

    Figure Lengend Snippet: PAK2 mediates cell cluster formation via E-cadherin phosphorylation. a , b GSEA showing the association between PAK2 and the adherens junction pathway. c , d Immunoblot analysis of E-cadherin and phosphorylated E-cadherin (Ser840) in BT549 cells after PAK2 overexpression or knockdown

    Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

    Techniques: Phospho-proteomics, Western Blot, Over Expression, Knockdown

    PAK2 interacts and colocalizes with E-cadherin. a Protein docking map and binding site of PAK2 for E-cadherin. The yellow color represents PAK2, and the blue color represents E-cadherin. b IF staining illustrating the colocalization of PAK2 and E-cadherin in MCF10A and BT549. c Co-IP analysis showing the association between PAK2 and E-cadherin in breast cancer cells

    Journal: Breast Cancer Research : BCR

    Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

    doi: 10.1186/s13058-025-02199-z

    Figure Lengend Snippet: PAK2 interacts and colocalizes with E-cadherin. a Protein docking map and binding site of PAK2 for E-cadherin. The yellow color represents PAK2, and the blue color represents E-cadherin. b IF staining illustrating the colocalization of PAK2 and E-cadherin in MCF10A and BT549. c Co-IP analysis showing the association between PAK2 and E-cadherin in breast cancer cells

    Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

    Techniques: Binding Assay, Staining, Co-Immunoprecipitation Assay

    FRAX597 inhibits PAK2 activity and CTC cluster formation. a CCK-8 assay evaluating the viability of BT549 after 48 h treatment with FRAX597. b Immunoblot analysis of total PAK2 and phosphorylated PAK2 protein levels in BT549 treated with 2.5 or 5 µM FRAX597 for 24 and 48 h. c Representative images and quantification of BT549 cell aggregation after 24 h FRAX597 treatment. d Immunoblot analysis of total and phosphorylated PAK2, and total and phosphorylated E-cadherin in BT549 treated with 2.5 or 5 µM FRAX597 for 24 h

    Journal: Breast Cancer Research : BCR

    Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

    doi: 10.1186/s13058-025-02199-z

    Figure Lengend Snippet: FRAX597 inhibits PAK2 activity and CTC cluster formation. a CCK-8 assay evaluating the viability of BT549 after 48 h treatment with FRAX597. b Immunoblot analysis of total PAK2 and phosphorylated PAK2 protein levels in BT549 treated with 2.5 or 5 µM FRAX597 for 24 and 48 h. c Representative images and quantification of BT549 cell aggregation after 24 h FRAX597 treatment. d Immunoblot analysis of total and phosphorylated PAK2, and total and phosphorylated E-cadherin in BT549 treated with 2.5 or 5 µM FRAX597 for 24 h

    Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

    Techniques: Activity Assay, CCK-8 Assay, Western Blot

    FRAX597 suppresses breast cancer progression. a – c Effects of FRAX597 on BT549 cell proliferation, migration and invasion. d Schematic diagram of in vivo experimental design. e , f Tumor volume, tumor weight and body weight measurements of nude mice treated with FRAX597

    Journal: Breast Cancer Research : BCR

    Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

    doi: 10.1186/s13058-025-02199-z

    Figure Lengend Snippet: FRAX597 suppresses breast cancer progression. a – c Effects of FRAX597 on BT549 cell proliferation, migration and invasion. d Schematic diagram of in vivo experimental design. e , f Tumor volume, tumor weight and body weight measurements of nude mice treated with FRAX597

    Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

    Techniques: Migration, In Vivo